Zeta’s T/B Cell Cocktail – Dual Stain.
Zeta’s T/B Cell Cocktail is a dual stain recombinant antibody cocktail that provides dual expression of CD3 and CD20, which is used by pathologists to evaluate lymphoid populations in tissue sections. It allows for the simultaneous identification and spatial assessment of T cells (CD3⁺) and B cells (CD20⁺), which is critical in diagnosing and classifying a wide range of hematolymphoid and inflammatory conditions.
CD3 is a pan-T-cell marker found on all mature T lymphocytes (membrane and cytoplasmic expression). The recombinant antibody ZR414 recognizes the epsilon chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta). The CD3 antigen is a highly specific marker for T cells and has been reported expressed by most T cell neoplasms.
CD20 is B-cell marker expressed on mature B lymphocytes, but not plasma cells. CD20 is a non-Ig differentiation antigen of B cells and the expression of CD20 is restricted to normal and neoplastic B cells, being absent from all other leukocytes and tissues. CD20 is the most specific B-cell marker used in paraffin immunohistochemistry.
Clinically, Zeta’s T/B Cell Cocktail can help differentiates between B-cell and T-cell lymphomas, which have distinct prognoses and treatments and help identify mixed or composite lymphomas and detect aberrant marker loss or co-expression. Additionally, the T/B Cell Cocktail can support confirmation of monoclonality vs. polyclonal reactive infiltrates: (1) Reactive contains mixed CD3⁺ and CD20⁺ cells and (2) Lymphoma has a dominant population of one cell type. In solid organ pathology (e.g., colon, breast, skin), the T/B Cell Cocktail can assess, Tumor-infiltrating lymphocytes (TILs), Chronic inflammation (e.g., autoimmune, viral), and Immune response patterns in transplant rejection.
Zeta’s T/B Cell Cocktail combines L26 (red) and ZR414 (brown) and uses two different chromogens to allow distinct visual separation on a single slide.
Zeta’s DUAL STAIN products provide more information from limited tissues, by seeing two related markers on the same slide while also allowing pathologists to see cellular localization and interaction within the tumor environment. Zeta uses one mouse and one rabbit recombinant monoclonal in each cocktail to allow for differential color results.
Zeta Corporation is a leader in recombinant monoclonal IVD antibodies for immunohistochemistry (IHC) offering 400+ tumor and tissue specific relevant products developed by a pathologist for pathologists. Visit Precision Recombinant Antibodies – IVD Antibodies – About Zeta Corporation (zeta-corp.com) to learn more about Zeta Corporation.