Recognizes a protein of 51kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified based on homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing’s Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22) (q24;q12), which results in the fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues, only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in most vascular tumors, including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi’s Sarcomas. The high sensitivity and specificity of Fli-1 equal or exceed that of the established vascular markers like CD31, CD34, and Factor VIII.