The BRAF gene encodes a protein that is part of the RAS-RAF-MEK-ERK signaling pathway, which regulates cell division and proliferation. The V600E mutation in the BRAF gene leads to the production of a constitutively active BRAF protein, resulting in uncontrolled cell growth and division. Identifying the presence of this mutation is crucial for diagnosing and guiding the treatment of certain cancers. The BRAF (V600E) antibody is used in immunohistochemistry to detect the presence of a specific mutation in the BRAF gene known as V600E. This mutation is commonly associated with various cancers, including melanoma, colorectal cancer, and certain types of thyroid cancer, lung cancer, and Hairy cell leukemia. The BRAF (V600E) antibody specifically binds to the mutated BRAF protein, allowing pathologists to detect the mutation in cancer tissue. Immunohistochemistry using this antibody is often employed in the evaluation of tumor specimens to aid in the diagnosis and classification of cancers.