Zeta’s rabbit recombinant monoclonal antibody recognizes p53, a 53kDa protein, which is identified as the p53 suppressor gene product. Zeta’s p53 antibody reacts with the mutant as well as the wild form of p53 protein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. p53 binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with the p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.