Zeta’s rabbit recombinant monoclonal antibody recognizes NSE-gamma, a protein of about 50kDa which is identified as gamma-enolase. There are three isoenzymes of enolases that have been identified: alpha, beta and gamma. Alpha-isoform is expressed in most tissues; beta-form is expressed predominantly in muscle tissue; and gamma-enolase is found only in nervous tissue. The NSE isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It is typically used in combination with other markers such as synaptophysin, Chromogranin A, and Neurofilament.