Zeta Universal HRP Polymer Detection

A primary antibody specific to an antigen on a formalin-fixed paraffin-embedded (FFPE) tissue section is detected by the Zeta Universal HRP Polymer Detection Kit.   The antigen sites are then visualized with DAB chromogen/substrate.  The reagent is ready-to-use in a convenient dropper bottle.  The Zeta Universal HRP Polymer Detection Kit is a one-step system which uses a direct method resulting in a polymer-secondary antibodies-HRP complex that universally detects primary mouse and rabbit antibodies.  The resulting chromogenic reaction can be visualized by HRP-compatible chromogens using light microscopy.

Zeta Corporation offers polymer-based detection to reduce non-specific staining and increase the signal-to-noise ratio of the immunohistochemistry application (IHC).  Many of the human organs contain endogenous biotin that might interfere with the actual staining for IHC and require additional blocking steps. With the advent of polymer detection technology, the biotin was completely removed from the detection chemistry and thus reducing the “background” noise.  However, the size of the polymer molecule also introduced the steric hindrance.

Therefore, Zeta ensured that our detection polymers are small in size to avoid steric hindrance and help increase the sensitivity and specificity of the detection process.