MGMT

    Neuropathology/Endocrine
    ZM314 Mouse Monoclonal

    Zeta MGMT Antibody.  Zeta’s recombinant mouse antibody recognizes MGMT, a DNA repair protein that reverses DNA damage induced by alkylating agents and acts as a suppressor of mutation and carcinogenesis.  Methylation of of the promoter sequence of the MGMT gene silences the MGMT, making tumor cells more sensitive to treatment with cancer chemotherapeutic alkylating agents, which act by inducing the formation of lethal cross‐links at the O6‐ alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6‐position of guanine in DNA (prior to cross‐link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA‐damage toleration. Tumors with high levels of MGMT are likely to be drug resistant.

    MGMT immunohistochemistry (IHC) is being evaluated in many studies as a biomarker for chemotherapy treatments, and for example, may have validitity as a biomarker for response to combination therapy with capecitabine and temozolomide in patients with advanced neuroendocrine carcinomas.

    Specifications
    Species Reactivity:Humans; others not tested
    Known Applications:Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
    Supplied As:Buffer with protein carrier and preservative
    Storage:Store at 2ºC to 8ºC
    Control:Colon carcinoma
    Visualization:Nuclear
    Isotype:IgG1 /κ
    Immunogen:Purified recombinant human MGMT protein
    Ordering Information
    Classification IVD
    Catalogue Number Z2618ML
    Classification IVD
    Catalogue Number Z2618ML
    Package Inserts
    IFU-MGMT ZM314
    Regulatory Notice
    Product classification varies based on regulations of individual countries. Please contact your local distributor for more information. Products labeled as ASR or RUO in the US might be available as IVD or RUO in respective countries.
    SDS
    SDS