Human colon carcinoma stained with anti-Cyclin D1 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells.
Cyclin E belongs to the highly conserved cyclin family; whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
Specifications
Species Reactivity:Humans; others not tested
Known Applications:Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Supplied As:Buffer with protein carrier and preservative
Storage:Store at 2ºC to 8ºC
Control:Colon carcinoma
Visualization:Nuclear
Isotype:IgG2b /κ
Immunogen:Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176)
Ordering Information
Package Inserts
SDS