Human urothelial carcinoma stained with anti-thymidylate synthase (TS) antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), essential for DNA biosynthesis. TS is also a critical target for fluoropyrimidines, an essential antineoplastic drug widely used to treat solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP, which inactivates TS by forming a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Specifications
Species Reactivity:Humans; others not tested
Known Applications:Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Supplied As:Buffer with protein carrier and preservative
Storage:Store at 2ºC to 8ºC
Control:5-FU resistant cancer
Visualization:Cytoplasmic
Isotype:IgG1
Immunogen:Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)
Ordering Information
Package Inserts
IFU-Thymidylate Synthase (TS) ZR245
SDS