Human melanoma stained with anti-S-100 antibody using peroxidase0conjugate and DAB chromogen. Note intense nuclear and cytoplasmic staining of melanoma cells.
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. Antibody to S100 stains Schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. Ab-1 is excellent for immunohistochemical staining of formalin-fixed, paraffin embedded tissues. S100 protein is highly soluble and may be eluted from frozen tissue during staining tissues.
Specifications
Species Reactivity:Humans; others not tested
Known Applications:Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Supplied As:Buffer with protein carrier and preservative
Storage:Store at 2ºC to 8ºC
Control:Melanoma
Visualization:Cytoplasmic and nuclear
Isotype:IgG2a /κ
Immunogen:Purified bovine brain S100 p
Ordering Information
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