Preferred Clone
Human tonsil stained with anti-retinoblastoma (Rb) antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of follicular center B cells.
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross-reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell, and cervical carcinoma.
Specifications
Species Reactivity:Humans; others not tested
Known Applications:Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Supplied As:Buffer with protein carrier and preservative
Storage:Store at 2ºC to 8ºC
Control:Colon cancer
Visualization:Nuclear
Isotype:IgG1 /κ
Immunogen:Human Rb1 protein fragment of 283 amino acid residues
Ordering Information
Package Inserts
SDS