preferred clone
Formalin-fixed, paraffin-embedded human colon carcinoma (Lynch) stained with anti-MSH-6 antibody using peroxidase-conjugate and DAB chromogen. Note the absence of nuclear staining of tumor cells whereas normal lymphocytes and stromal cells are positive.
The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in understanding the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.
Specifications
Species Reactivity:Humans; others not tested
Known Applications:Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Supplied As:Buffer with protein carrier and preservative
Storage:Store at 2ºC to 8ºC
Control:Colon carcinoma
Visualization:Nuclear
Isotype:IgG
Immunogen:Recombinant fragment (around aa 374-540) of human MSH6 protein
Ordering Information
Package Inserts
SDS