Human colon adenocarcinoma with Lynch syndrome stained with anti-MSH-2 antibody using peroxidase-conjugate and DAB chromogen. Note absence of nuclear staining of tumor cells whereas the background cells are positive.
Germline mutations in human mismatch repair genes (hMSH2, hMSH6, hMLH1, hPMS2) account for majority of the hereditary non-polyposis colorectal carcinoma (HPNCC). CpG dinucleotides in the hMSH2 and hMLH1 genes are hotspots for HNPCC mutations. These mutations cause a mismatch repair deficiency resulting in a mutator phenotype where the replication errors are not repaired. Microsatellites / simple repetitive sequences are prone to this type of replication errors and instability of these microsatellites correlates with the occurrence of HPNCC. hMSH2 binds to another MutS homolog protein GTBP to form a heterodimeric complex called hMutS beta, which binds to insertion/deletion loops in DNA.
Specifications
Species Reactivity:Humans; others not tested
Known Applications:Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Supplied As:Buffer with protein carrier and preservative
Storage:Store at 2ºC to 8ºC
Control:Colon carcinoma
Visualization:Nuclear
Isotype:IgG1 /κ
Immunogen:BALB/C mice were injected with recombinant human MSH2 protein
Ordering Information
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