BRAF (V600E)

    Dematopathology
    ZR6 Rabbit Monoclonal

    Zeta’s rabbit recombinant monoclonal antibody specifically recognizes the protein encoded by the BRAF (V600E) mutation. The V600E mutation leads to the production of a constitutively active BRAF protein, resulting in uncontrolled cell growth and division. Identifying the presence of this mutation is crucial for diagnosing and guiding the treatment of melanoma, colorectal cancer, and certain types of thyroid cancer, lung cancer, and Hairy cell leukemia.

    The BRAF gene encodes a protein that is part of the RAS-RAF-MEK-ERK signaling pathway, which regulates cell division and proliferation. The V600E mutation in the BRAF gene leads to the production of a constitutively active BRAF protein, resulting in uncontrolled cell growth and division. Identifying the presence of this mutation is crucial for diagnosing and guiding the treatment of certain cancers. The BRAF (V600E) antibody is used in immunohistochemistry to detect the presence of a specific mutation in the BRAF gene known as V600E. This mutation is commonly associated with various cancers, including melanoma, colorectal cancer, and certain types of thyroid cancer, lung cancer, and Hairy cell leukemia. The BRAF (V600E) antibody specifically binds to the mutated BRAF protein, allowing pathologists to detect the mutation in cancer tissue. Immunohistochemistry using this antibody is often employed in the evaluation of tumor specimens to aid in the diagnosis and classification of cancers.

    In BRAF (V600E) melanoma, the upstream RAS kinase activity is not required for cellular division, indicating BRAF (V600E) as a distinct and defining driver of malignant disease progression in melanoma.  Although BRAF (V600E) mutation in malignant melanoma is prevalent, the BRAF gene encoding the V600E mutant has been identified in other solid tumors such as thyroid cancer, bladder urothelial cancer, chronic lymphocytic leukemia, colorectal cancer, glioblastoma multiforme (GBM), head and neck squamous cell carcinoma, renal papillary cell carcinoma, hepatocellular carcinoma, lung adenocarcinoma, and lymphoma.

    The BRAF (V600E) Zeta-Antibody ZR6 recognizes the mutated valine V600 changed to the highly polar and acidic glutamate E sidechain -CH2-CH2-COO typically physiologically ionized.   Antibody clone ZR6 represents a new and favorable option in the detection of BRAF (V600E) in malignant melanoma and other malignancies.

    Zeta Corporation is a leader in recombinant monoclonal IVD antibodies for immunohistochemistry (IHC) offering 400+ tumor and tissue specific relevant products developed by a pathologist for pathologists.

     

    Specifications
    Species Reactivity:Humans; others not tested
    Known Applications:Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
    Supplied As:Buffer with protein carrier and preservative
    Storage:Store at 2ºC to 8ºC
    Control:Melanoma with V600E mutation
    Visualization:Nuclear
    Isotype:IgG
    Immunogen:A peptide corresponding to BRAF V600E mutant
    Ordering Information
    Classification IVD
    Catalogue Number Z2811RL
    Classification IVD (Non-EU)/ RUO (EU)
    Catalogue Number Z2811RL
    Package Inserts
    IFU-BRAF ZR6- IVD
    Regulatory Notice
    Product classification varies based on regulations of individual countries. Please contact your local distributor for more information. Products labeled as ASR or RUO in the US might be available as IVD or RUO in respective countries.
    IFU-BRAF ZR6 - RUO
    Regulatory Notice
    Product classification varies based on regulations of individual countries. Please contact your local distributor for more information. Products labeled as ASR or RUO in the US might be available as IVD or RUO in respective countries.
    SDS
    SDS